Digital Chromatography and the Forma- Tion of Heterogeneous Droplet Libraries Using Microfractionation in Droplets (μfd)
نویسندگان
چکیده
Generating chemical libraries in droplet form is vital to the adoption of microdroplet systems in high-throughput screening (HTS). This paper presents microfractionation in droplets (μFD), a method for generating heterogeneous droplet libraries using standard separation processes. In μFD, compounds eluting from a separation column are permanently encapsulated into droplets by a droplet generator placed immediately after the column. A prototype, comprised of a C18 cartridge and a cross-junction droplet generator, creates a library of organic dyes. Cascaded fiberoptic absorbance detectors generate “digital” chromatograms which report on each droplet’s size, frequency, and composition. This technique can be adapted to many separation processes to form a variety of droplet libraries.
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